Quantitative determination method for target particles, photometric analysis device, and computer program for photometric analysis

摘要

The method of the present invention includes: preparing a sample solution containing the target particles and luminescent probes to be bound to the target particles, and binding these in the sample solution; moving a position of a light detection region of the optical system in the sample solution using a confocal microscope or a multiphoton microscope, and detecting light signal emitted from the luminescent probe in the light detection region while moving the position of the light detection region, and individually detecting the target particles directly or indirectly; and counting the number of the detected target particles, and calculating the concentration of the target particles in the sample solution from the number of the counted target particles on the basis of a calibration curve that approximates the correlation between the concentration or quantity of the target particles in the sample solution and the number of the target particles.

主权项

1. A method for quantifying target particles which are dispersed and moving at random in a sample solution, the method comprising:
(a) preparing a sample solution containing the target particles and luminescent probes to be bound to the target particles, and binding the target particles and the luminescent probes in the sample solution; (b) moving a position of a light detection region of an optical system in the sample solution with use of an optical system of a confocal microscope or a multiphoton microscope, and detecting a light signal emitted from the luminescent probes in the light detection region while moving the position of the light detection region of the optical system in the sample solution, and individually detecting the target particle directly or indirectly; and (c) counting, using a processor, the number of the target particles detected in the (b) and calculating, using the processor, the concentration of the target particles in the sample solution from the number of the counted target particles on the basis of a calibration curve that approximates the correlation between the concentration or quantity of the target particles in the sample solution and the number of the target particles.