Methods and reagents for reducing non-specific amplification

摘要

The present invention provides methods and reagents for use in the amplification of nucleic acids. Amplification carried out using oligonucleotides containing modified nucleotides can result in less non-specific amplification compared to amplification carried out using unmodified oligonucleotides.

主权项

1. A method of reducing or preventing non-specific amplification of nucleic acid during a multiplex amplification reaction comprising providing multiple pairs of primer oligonucleotides capable of amplifying multiple target nucleic acid sequences; providing multiple probe oligonucleotides, each probe oligonucleotide incorporating a N2-benzyl-dG nucleotide that blocks the extension of a primer oligonucleotide by a thermostable DNA polymerase by more than 2 nucleotides beyond the position of the N2-benzyl-dG-nucleotide if the primer oligonucleotide hybridizes to the probe oligonucleotide.