Fluorescence assay for ghrelin O-acyltransferase activity

摘要

An assay to detect ghrelin O-acyltransferase activity using an acrylodan-labeled peptide mimic of ghrelin that provides for high-throughput screening for ghrelin O-acyltransferase inhibitors and detection via high performance liquid chromatography. Alternatively, the assay for ghrelin acylation may be based on a synthetic peptide substrate that mimics the N-terminal sequence of ghrelin and has an environmentally-sensitive fluorophore attached to its C-terminal amino acid through chemoselective ligation.

主权项

1. A substrate for use in an assay for ghrelin acylation, comprising:
an isolated peptide having the sequence selected from the group consisting of SEQ. ID NO: 4 and SEQ. ID NO: 5.