Method for culturing cells on culture medium

摘要

A method for culturing a number of cells includes the following steps. A culture medium is provided. The culture medium has a carbon nanotube structure and a hydrophilic layer. The hydrophilic layer is formed on a surface of the carbon nanotube structure. A polar layer is formed on a surface of the hydrophilic layer away from the carbon nanotube structure. The cells are seeded and cultured on the polar layer.

主权项

1. A method for culturing a plurality of cells, comprising:
(1) providing a culture medium having a carbon nanotube structure and a hydrophilic layer formed on a surface of the carbon nanotube structure, wherein a material of the hydrophilic layer is an inorganic material; (2) forming a polar surface on a surface of the hydrophilic layer away from the carbon nanotube structure; (3) directly seeding a plurality of cells on said polar surface of the hydrophilic layer, wherein the plurality of cells are in contact with the hydrophilic layer; and (4) culturing the plurality of cells; wherein said forming a polar surface comprises: (2a) applying a polyamino acid solution on the surface of the hydrophilic layer away from the carbon nanotube structure, and completely purging the polyamino acid solution from the hydrophilic layer; or, in the alternative, (2b) applying a polyetherimide solution on the surface of the hydrophilic layer away from the carbon nanotube structure, and completely purging the polyetherimide solution from the hydrophilic layer.