Genetically modified mice having humanized TCR variable genes

摘要

The invention provides a genetically modified non-human animal that comprises in its genome unrearranged T cell receptor variable gene loci, as well as embryos, cells, and tissues comprising the same. Also provided are constructs for making said genetically modified non-human animal and methods of making the same. Various methods of using the genetically modified non-human animal are also provided.

主权项

1. A genetically modified mouse whose genome comprises:
a replacement of an endogenous T cell receptor (TCR) variable α (Vα)gene with unarranged human TCR Vα segments and unrearranged human TCR Jα gene segments, wherein the unrearranged human TCR Vα and Jα gene segments are operably linked to a mouse TCR constant α (Cα) gene.