Identification of antibodies specific for lyssaviruses and methods of their use

摘要

Described herein is a method of identifying a monoclonal antibody (or antigen-binding fragment thereof) that specifically binds a plurality of lyssaviruses for use in post-exposure rabies prophylaxis or in the treatment of clinical rabies. The method includes using a naïve antibody phage display library to screen for phage clones that bind whole recombinant rabies virus or cells expressing glycoprotein from multiple lyssaviruses (such as RABV, MOKV and WCBV) and/or specifically bind recombinant glycoprotein from different lyssaviruses.

主权项

1. A method of identifying a monoclonal antibody, or antigen-binding fragment thereof, that specifically binds at least two different lyssavirus species, comprising:
screening a naïve antibody phage display library with at least two different cell lines, wherein each cell line expresses a different lyssavirus glycoprotein selected from the RABV glycoprotein, the MOKV glycoprotein, the WCBV glycoprotein, the LBV glycoprotein and the DUVV glycoprotein, and wherein the phage display library is screened sequentially on the at least two different cell lines; and selecting a phage display clone that specifically binds to at least two different lyssavirus species, glycoproteins from at least two different lyssavirus species, or both, thereby identifying a monoclonal antibody, or antigen-binding fragment thereof, that specifically binds at least two different lyssavirus species.