Nanotopography-mediated reverse uptake platform for nucleic acid delivery and applications thereof

摘要

This application discloses a nanotopography-mediated reverse uptake (NanoRU) platform useful for intracellular delivery of nucleic acids into mammalian cells, in particular stem cells, as well as methods of preparation and applications thereof. In particular, this system can be used to deliver small interfering ribonucleic acids (siRNAs) into neural stem cells and enhance neuronal differentiation of the stem cells.

主权项

1. A nucleic acid delivery system comprising at least one nucleic acid, a self-assembled silicon oxide (silica) nanoparticle (SiNP) monolayer, and a film comprising one or more of extracellular matrix (ECM) proteins, wherein the silicon oxide nanoparticle monolayer is coated with the film, wherein:
the film has topographical features capable of facilitating delivery of the at least one nucleic acid into cells; the topographical features enable cellular uptake of the at least one nucleic acid without cellular uptake of the silicon oxide nanoparticle monolayer; and the sizes of silica nanoparticles are in the range of 50 nm to 700 nm.