Structurally modified acellular tissue engineering scaffolds and methods of production

摘要

Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.

主权项

1. A bioscaffold produced by the process of chemically treating a natural animal tissue with simultaneous use of an oxidant and detergent,
wherein an extracellular matrix component of said bioscaffold has an average porosity that is at least 20% greater than the average porosity of said extracellular matrix component of said natural animal tissue prior to said treating, wherein the average porosity that is at least 20% greater results from controlled dissolving of the interstitial structure of the bioscaffold with said simultaneous use of an oxidant and detergent; and wherein said natural animal tissue is selected from the group consisting of muscle, tendon, nerve, bone, meniscus, cartilage, intervertebral discs, and ligament tissue.