SELF-POWERED BLOOD COAGULATION CHIP FOR INR VALUE AND HEMATOCRIT DETERMINATION

摘要

A point-of-test diagnostic chip that is capable of simultaneous evaluation of blood coagulation time (INR Value) and hematocrit level. A finger prick volume of blood is placed at the inlet of the device, and the residual vacuum contained within the device provides a pressure gradient to drive the flow of whole blood. The INR value is determined by the distance the blood traverses down the channel before it coagulates. The channel is primed with tissue factor and phospholipids to induce coagulation. A control channel is primed with anticoagulants for use as a way to normalize initial loading times. The hematocrit level is determined by sedimentation of blood cells into a trench and the interface between blood cells that have overfilled the trench and plasma provide a visual readout.

主权项

1. An apparatus for measuring one or more physiological characteristics of a blood sample, the apparatus comprising:
a substrate; a hematocrit measurement channel disposed within the substrate; the hematocrit measurement channel comprising a first closed end and a second end coupled to an orifice for receiving the blood sample; and a plasma skimming trench between the second end of the hematocrit measurement channel and the orifice; wherein the plasma skimming trench is configured to skim plasma from the blood sample flowing into the hematocrit measurement channel to generate a visible interface between the skimmed plasma and the blood sample; and wherein the location of the interface when the blood sample is fully loaded in said hematocrit measurement channel is indicative of the hematocrit level of the blood sample.