| 摘要 |
FIELD: medicine.SUBSTANCE: technique involves sampling a biopsy material from a skin growth of interest, carrying out real-time PCR of the sampled material to measure a relative microRNA expression level as shown by snRNA-U6 normalising microRNA with hsa-miR-205 gene taken as a differential diagnostic marker. If the relative expression level falls within the range of 0.29926 to 13.202, the analysing skin growth is considered to be melanoma; the relative expression level from 25.70993 to 1583.114 shows a nevus; an intermediate range of values falling within 13.202 to 25.70993 requires performing an immunohistochemical study of the biopsy material with using primary antibodies against the specific markers: S 100, HMB 45, MART 1, MELAN A, CD-63, MITF.EFFECT: using the invention enables optimising and accelerating the differential diagnosis of melanocytic skin growths.1 tbl, 3 ex |
