| 摘要 |
Provided is a method for measuring cholesterol in high-density lipoprotein (HDL) simply and accurately. A method for measuring cholesterol in high-density lipoprotein in a specimen, comprising reacting the specimen with i) a cholesterol ester hydrolase and a cholesterol oxidase, or ii) a cholesterol ester hydrolase, an oxidized coenzyme, and a cholesterol dehydrogenase, in an aqueous medium comprising at least one substance selected from the group consisting of pyridinium salts and quaternary ammonium salts each having a specific structure, and a polyanion to form hydrogen peroxide or reduced coenzyme and measuring the formed hydrogen peroxide or reduced coenzyme. |
| 主权项 |
1. A method for measuring cholesterol in high-density lipoprotein in a specimen, comprising reacting the specimen with i) a cholesterol ester hydrolase and a cholesterol oxidase, or ii) a cholesterol ester hydrolase, an oxidized coenzyme, and a cholesterol dehydrogenase, in an aqueous medium comprising at least one substance selected from the group consisting of a substance represented by formula (I): (wherein R1 represents a substituted or unsubstituted alkyl or alkenyl having 8 to 14 carbon atoms; Ra represents a hydrogen atom, a substituted or unsubstituted alkyl, or a substituted or unsubstituted alkenyl; n represents an integer of 1 to 5; and X− represents a monovalent anion) and a substance represented by formula (II): (wherein R2 represents a substituted or unsubstituted alkyl or alkenyl having 6 to 30 carbon atoms; R3 represents a substituted or unsubstituted alkyl having 1 to 6 carbon atoms or alkenyl having 2 to 6 carbon atoms; R4 represents a hydrogen atom, substituted or unsubstituted aryl, or substituted or unsubstituted alkyl; and Y− represents a monovalent anion), and a polyanion to form hydrogen peroxide or a reduced coenzyme; and measuring the formed hydrogen peroxide or reduced coenzyme. |
