| 摘要 |
An acetyl-CoA producing microorganism obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism that does not have any of the following (a), (b), (c), (d) or (e): (a) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate; (b) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and CO2 to pyruvate; (c) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and CO2 to ethylmalonyl-CoA or glutaconyl-CoA; (d) a carbon dioxide fixation cycle including an enzymatic reaction from CO2 to formate; or (e) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase. |
| 主权项 |
1. An acetyl-CoA producing microorganism comprising an acetyl-CoA production cycle obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism that does not have any of the following (a), (b), (c), (d) or (e):
(a) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate; (b) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and CO2 to pyruvate; (c) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and CO2 to ethylmalonyl-CoA or glutaconyl-CoA; (d) a carbon dioxide fixation cycle including an enzymatic reaction from CO2 to formate; or (e) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase, the acetyl-CoA producing microorganism being obtained without imparting any of (a), (b), (c), or (d) to the microorganism, or such that the microorganism exhibits none of the functions of (a), (b), (c), or (d) even if one or more of (a), (b), (c), or (d) is imparted thereto, wherein, in the microorganism: at least one enzymatic activity selected from the group consisting of pyruvate kinase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, phosphoenolpyruvate carboxykinase, malate dehydrogenase, malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, hydroxypyruvate isomerase, hydroxypyruvate reductase, glycerate 2-kinase, glycerate 3-kinase, phosphoglycerate mutase, and enolase, is enhanced; at least one enzymatic activity selected from the group consisting of a malic enzyme and fumarate reductase is inactivated or reduced; or any combination thereof. |
