发明名称 |
HEAVY METAL REDUCTION IN PLANTA |
摘要 |
There is described a mutant, non-naturally occurring or transgenic plant or plant cell comprising (a) a polynucleotide selected from the group consisting of: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 71% sequence identity to SEQ ID NOs: 1, 2, 27, 28 or 29 or 51; or (ii) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 65% sequence identity to any of SEQ ID NOs: 3 to 23 or 30 to 50; or (iii) a polynucleotide encoding a NtMRP polypeptide comprising, consisting or consisting essentially of a sequence having at least 65% sequence identity to any of SEQ ID NOs. 24 to 26 or 52, and wherein the polypeptide has heavy metal transporter activity; or (b) a polynucleotide construct of at least 15 contiguous nucleotides in length that is at least 65% identical to a region of any of SEQ ID NOs: 1 to 23 or 27 to 51; or (c) a double-stranded RNA comprising at least two sequences that are at least partially complementary to each other and wherein a sense strand comprises a first sequence and an antisense strand comprises a second sequence and wherein at least one of the sequences comprises at least 10 contiguous nucleotides of NtMRP RNA; or (d) an expression vector comprising the polynucleotide as set forth in (i), (ii) or (iii) or the polynucleotide construct as set forth in (b). |
申请公布号 |
US2015232867(A1) |
申请公布日期 |
2015.08.20 |
申请号 |
US201113819063 |
申请日期 |
2011.08.31 |
申请人 |
Bovet Lucien |
发明人 |
Bovet Lucien |
分类号 |
C12N15/82;C12N15/113;C07K16/16;C12Q1/68;C07K14/415 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
1. A mutant, non-naturally occurring or transgenic plant or plant cell comprising
(a) a polynucleotide selected from the group consisting of:
(i) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 71% sequence identity to SEQ ID NOs: 1, 2, 27, 28 or 29 or 51; or(ii) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 65% sequence identity to any of SEQ ID NOs: 3 to 23 or 30 to 50; or(iii) a polynucleotide encoding a NtMRP polypeptide comprising, consisting or consisting essentially of a sequence having at least 65% sequence identity to any of SEQ ID NOs. 24 to 26 or 52, and wherein the polypeptide has heavy metal transporter activity; or (b) a polynucleotide construct of at least 15 contiguous nucleotides in length that is at least 65% identical to a region of any of SEQ ID NOs: 1 to 23 or 27 to 51; or (c) a double-stranded RNA comprising at least two sequences that are at least partially complementary to each other and wherein a sense strand comprises a first sequence and an antisense strand comprises a second sequence and wherein at least one of the sequences comprises at least 10 contiguous nucleotides of NtMRP RNA; or (d) an expression vector comprising the polynucleotide as set forth in (i), (ii) or (iii) or the polynucleotide construct as set forth in (b). |
地址 |
La-chaux-de-Fonds CH |