METHOD AND APPARATUS FOR MULTI COLUMN CHROMATOGRAPHIC PURIFICATION

摘要

A method for separating a feed mixture comprising at least two solutes by an intermittent multi column chromatography process with a basic step N performed in a predetermined switching time period. Basic step N comprises two sub steps which are carried out sequentially according to a predetermined step ratio. In sub step one inlet ports and outlet ports are open, a desorbent and a feed mixture enters upstream of a first and a third section. A first outlet stream comprising the desorbent and at least one solute is withdrawn downstream of the first section a second outlet stream comprising the desorbent and at least a further solute is withdrawn downstream of a the third section. In sub step two inlet ports and outlet ports are closed, a fluid stream comprising the desorbent part of the feed mixture circulates through three sections in a closed loop.

主权项

1. A method to separate a feed mixture (F) comprising at least one solvent and two solutes by an intermittent multi column chromatography process with a basic step N performed in a predetermined switching time period (t*), basic step N comprises two sub steps which are carried out sequentially according to a predetermined step ratio (α)
in sub step one inlet ports (IP1, IP3) and outlet ports (OP1, OP3) are opened, a desorbent (D) enters upstream of a first section (I) through IP1, the feed mixture (F) enters upstream of a third section (III) through IP3, a first outlet stream (E) comprising the desorbent (D) and at least one solute is withdrawn downstream of the first section (I) through OP1, a second outlet stream (R) comprising the desorbent (D) and at least a further solute is withdrawn downstream of the third section (III) through OP3; in sub step two inlet ports (IP1, IP2, IP3) and outlet ports (OP1, OP2, OP3) are closed, a fluid stream comprising the desorbent (D) and part of the feed mixture (F) is circulated through three sections (Γ to III′) in a closed loop.