| 摘要 |
PROBLEM TO BE SOLVED: To provide compositions for partial or complete inactivation of a GS gene, and methods of making and using the compositions (reagents), e.g., to inactivate GS in a cell to produce cell lines in which a GS gene is inactivated, where GS disrupted cell lines are useful, e.g., in production of recombinant proteins.SOLUTION: Disclosed herein are methods and compositions for inactivating a glutamine synthetase (GS) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding the fusion proteins are also provided, as are cells comprising the polynucleotides and the fusion proteins. |
