NOVEL LACCASE FROM GANODERMA LUCIDUM CAPABLE OF ENHANCING ENZYMATIC DEGRADATION OF LIGNOCELLULOLYTIC BIOMASS

摘要

The invention addresses the need for enzymes that can enhance the yield of fermentable sugar from the hydrolysis of lignocellulose biomass, for example sugar cane bagasse, barley straw and wheat straw, such that the use of this biomass can become economically viable. The invention provides methods for the hydrolysis of biomass using a laccase derived from Ganoderma lucidum. Further, the invention provides an enzyme composition comprising a laccase derived from Ganoderma lucidum which may be combined with one or more cellulases, and for its use in enhancing lignocellulose biomass hydrolysis.

主权项

1. A method for enhancing enzymatic hydrolysis of lignocellulose biomass, comprising the steps of:
a. providing an aqueous dispersion of biomass; b. adding a preparation of fungal laccase to the biomass (a); c. adding a preparation of one or more cellulose hydrolysing enzyme to the biomass (b), wherein the addition in step b) is either simultaneous with the addition in step c), or is prior to the addition in step c); d. incubating the biomass of step b) and step c) either simultaneously or in sequence;
wherein the fungal laccase is a polypeptide having at least 85% amino acid sequence identity to SEQ ID NO: 5.