Thermostable Type-A DNA Polymerase Mutant with Increased Resistance to Inhibitors in Blood

摘要

The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.

主权项

1. An isolated, mutant thermostable Type-A DNA polymerase comprising:
a first mutation at residue 507 of a wild-type Taq DNA polymerase or at a residue corresponding to residue 507 of the wild-type Taq DNA polymerase in another thermostable Type-A DNA polymerase; and additional mutations at residues 59, 155, 245, and 749 of the wild-type Taq DNA polymerase, or at corresponding residues in the another thermostable Type-A DNA polymerase, wherein the mutant polymerase possesses (i) DNA polymerase activity and (ii) a higher resistance to a polymerization activity inhibitor than the wild-type DNA polymerase from which the mutant polymerase is derived, and wherein the mutant polymerase is free of mutation at residue 375 or 734 of the wild-type Taq DNA polymerase, or at corresponding residues in the another thermostable Type-A DNA polymerase.