KITS AND METHODS FOR REPROGRAMING NON-HEPATOCYTE CELLS INTO HEPATOCYTE CELLS
摘要
A method for inducing reprograming of a cell of a first type which is not a non-hepatocyte (non-hepatocyte cell), into a cell with functional hepatic drug metabolizing and transporting capabilities, is disclosed. The non-hepatocyte is induced to express or overexpress hepatic fate conversion and maturation factors, cultured in somatic cell culture medium, hepatocyte cell culture medium and hepatocyte maturation medium for a sufficient period of time to convert the non-hepatocyte cell into a cell with hepatocyte-like properties. The iHeps induced according to the methods disclosed herein are functional induced hepatocytes (iHeps) in that they express I and II drug-metabolizing enzymes and phase III drug transporters and show superior drug metabolizing activity compared to iHeps obtained by prior art methods. The iHeps thus provide a cell resource for pharmaceutical applications.
申请公布号
WO2015120776(A1)
申请公布日期
2015.08.20
申请号
WO2015CN72232
申请日期
2015.02.04
申请人
PEKING UNIVERSITY;BEIJING VITALSTAR BIOTECHNOLOGY CO., LTD.;STEM CELL AND REGENERATIVE MEDICINE TRANSLATIONALRESEARCH INSTITUTE
发明人
DENG, HONGKUI;DU, YUANYUAN;SHI, YAN;JIA, JUN;WANG, JINLIN;XIANG, CHENGANG;SONG, NAN;XU, JUN;YIN, MING