| 摘要 |
FIELD: chemistry.SUBSTANCE: present invention relates to biotechnology and is an isolated version of Bacillus subtilisin, where said subtilisin version is a mature form having subtilisin activity and comprising a substitution at positions 118 and 213, where the numbering of the positions corresponds to the amino acid sequence of B. Amyloliquefaciens subtilisin BPN? with the sequence SEQ ID NO: 1,where said Bacillus subtilisin is FNA, and where said subtilisin version further comprises combinations of substitutions. The invention also relates to a method of cleaning fabric using said subtilisin version.EFFECT: invention enables to obtain subtilisin versions with a higher protein expression level.5 cl, 5 dwg, 4 tbl, 4 ex |
