Method of Producing Recombinant ADAMTS13 in Cell Culture

摘要

Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimeric vWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4*) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities. j~ 7 12 m33 cv) ~ ~ ~ IG IA0~ 1 9U