发明名称 |
GENETICALLY ENCODED FLUORESCENT SENSORS FOR DETECTING INTRACELLULAR SIGNALLING THROUGH DIACYLGLYCEROL PATHWAYS |
摘要 |
Described herein are novel fluorescent sensors for Diacyl Glycerol (DAG) and hosphatidylinositol 4,5-bisphosphate (PIP2) that are based on circularly permuted fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in amultiplex assay on standard fluorescent plate readers or live cell imaging systems. |
申请公布号 |
EP2825887(A4) |
申请公布日期 |
2015.08.19 |
申请号 |
EP20130761664 |
申请日期 |
2013.03.15 |
申请人 |
MONTANA MOLECULAR LLC |
发明人 |
HUGHES, THOMAS, E.;TEWSON, PAUL, H.;QUINN, ANNE, MARIE |
分类号 |
G01N33/542 |
主分类号 |
G01N33/542 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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