Multi-directional microfluidic devices and methods

摘要

Multi-directional microfluidic devices and methods for using the same are provided. Aspects of the invention include microfluidic devices that are configured to subject a sample to two or more directionally distinct flow fields, and include a separation medium and a binding medium, where the binding medium is in fluid communication with the separation medium. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.

主权项

1. A microfluidic device for detecting an analyte in a fluid sample, wherein the microfluidic device comprises a contiguous monolith comprising:
a separation medium having a separation flow path with a first directional axis; and a binding medium having a labeling flow path with a second directional axis, wherein the binding medium is in fluid communication with the separation medium, wherein the microfluidic device is configured to subject a sample to two or more directionally distinct flow fields coplanar with the separation medium.