Microfluidic devices and methods based on massively parallel picoreactors for cell and molecular diagnostics

摘要

Microfluidic devices and methods of forming cell reactors for performing cell analysis in a microfluidic chip. A microfluidic chip, in one implementation, includes a plurality of trapping sites, each of the plurality of trapping sites having a plurality of micropillars configured to trap one or more cells in an interior space formed by the plurality of micropillars. The plurality of micropillars in each trapping site form a picoreactor for cell and molecular diagnostics, such as characterizing, isolation, processing, and amplification of different cells, cells containing different substances, different particles, different biochemical compositions, proteins, and enzymes.

主权项

1. A method of forming cell reactors for performing cell analysis in a microfluidic chip, the method comprising:
trapping one or more cells in at least one of a plurality of trapping sites, each of the plurality of trapping sites having a plurality of micropillars configured to trap the one or more cells in an interior space formed by the plurality of micropillars; flowing a first immiscible fluid around the one or more cells to encapsulate the one or more cells at the plurality of trapping sites to form an encapsulated reactor array, after the trapping of the one or more cells.