Colorimetric gelatinase assay

摘要

The present invention is drawn toward a lateral-flow, colorimetric, gelatinase assay including a sample pad, a reagent pad, a membrane, and an absorbent pad, in that order, wherein the reagent pad has therein a dried form of gelatin-coated nanoparticles in a salt and sugar matrix, and wherein the membrane portion has a test stripe and a control stripe, such that the test stripe is a solution of electrolyte evaporated into a layer, and the control stripe is a solution of anti-gelatin antibody evaporated into a layer. The present invention is also drawn toward a method of detecting one or more gelatinases in a sample fluid using the assay, and methods of determining treatment for wound healing, cancer, ocular rosacea, periodontal disease and equine arthritis using the assay.

主权项

1. A lateral-flow, colorimetric, gelatinase assay comprising a sample pad, a reagent pad, a membrane, and an absorbent pad, in that order, wherein:
the reagent pad comprises therein a dried form of gelatin-coated nanoparticles in a salt and sugar matrix, and the membrane comprises a test stripe and a control stripe, wherein the test stripe comprises a solution of electrolyte evaporated into a layer, and the control stripe comprises a solution of anti-gelatin antibody evaporated into a layer.