| 主权项 |
1. A method for monitoring disappearance, regression, progression or recurrence of diseased tissue in a patient, the method comprising the steps of:
obtaining a first heterogeneous biological sample from the patient; generating a patient-specific genetic profile of diseased tissue by interrogating a plurality of genomic loci in genomic DNA and/or free DNA isolated from the first heterogeneous biological sample obtained from the patient for the presence and/or the amount of a plurality of biomarkers, wherein
(i) the plurality of genomic loci comprises at least one mutation in each of K-ras, adenomatous polyposis coli (APC), p53, and PIK3CA, and(ii) the presence of at least one mutation at an individual locus is indicative of the presence of diseased tissue; and initiating a treatment protocol after the first heterogeneous biological sample is obtained; obtaining a second heterogeneous biological sample from said patient after treatment; and monitoring the patient-specific genetic profile by interrogating the plurality of genomic loci in genomic DNA and/or free DNA isolated from the second heterogeneous biological sample for the presence and/or the amount of the plurality of biomarkers, wherein a change of the patient-specific genetic profile is indicative of the disappearance, regression, progression or recurrence of the diseased tissue in the patient. |
