Automated systems and methods for isolating regenerative cells from adipose tissue

摘要

A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer; introducing the adipose tissue into the vessel; introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided.

主权项

1. A mixing vessel for processing an adipose tissue to collect adipose derived regenerative cells, comprising:
at least one inlet port for receiving adipose tissue, a buffer and an enzyme solution; a centrally located cylinder comprising one or more nozzles configured to generate a high velocity fluid stream, wherein the nozzles are in an upward direction to generate an upward fluid stream and a jet mixing into the vessel to digest adipose tissue and form a digested buoyant fat layer and a non-buoyant aqueous layer comprising adipose derived regenerative cells; at least one outlet port comprising a filter unit, wherein the filter unit allows to pass the non-buoyant aqueous layer comprising adipose derived regenerative cells, wherein the cylinder is operably connected to the inlet port and the outlet port.