Device for concentrating and separating cells

摘要

Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.

主权项

1. A cell separation chip, comprising:
a planar substrate; a flow path formed on one side of the planar substrate and configured to allow a sample liquid containing cells to flow down; a cell concentration area provided in the flow path and configured to concentrate the cells; a cell arrangement area provided in the flow path and configured to arrange the cells; and a cell separation area provided in the flow path and configured to separate the cells, wherein the cell separation area comprises:
branched flow paths connected to the downstream end of the flow path, comprising an upper flow path and a lower flow path separated in the vertical direction of the flow path relative to the one surface of the planar substrate, wherein
the flow path is branched downstream of the cell concentration area,the sample liquid containing the cells passing through the cell concentration area is delivered into one of the branched flow paths, andthe cell arrangement area and the cell separation area are provided in the one of the branched flow paths; and wherein
the cell separation area is provided downstream of the cell arrangement area.