发明名称 |
Crosslinked hyaluronic acid in emulsion |
摘要 |
The present invention relates to methods of producing crosslinked hyaluronic acid microbeads, as well as the produced microbeads, said method comprising the steps of: (a) providing an aqueous alkaline solution comprising hyaluronic acid, or a salt thereof; (b) forming microdroplets having a desired size from the mixed solution of step (a) in an organic or oil phase to form a water in organic or water in oil (VWO) emulsion, wherein the amount of oil phase used is of from 20 to less than 50% by weight based on the sum of oil phase and water; (c) adding a solution comprising a crosslinking agent to the emulsion, whereby the reaction of hyaluronic acid with the crosslinking agent takes place to provide crosslinked hyaluronic acid microbeads; and (d) optionally working up the dispersion of crosslinked hyaluronic acid microbeads obtained in step (c). |
申请公布号 |
US9109051(B2) |
申请公布日期 |
2015.08.18 |
申请号 |
US200812809809 |
申请日期 |
2008.12.10 |
申请人 |
EVONIK GOLDSCHMIDT GMBH;NOVOZYMES BIOPOLYMER A/S |
发明人 |
Wenk Hans Henning;Farwick Mike;Nattland Sandra;Maczkiewitz Ursula;Moelholm Malle Birgitte |
分类号 |
C08B37/08;A61K8/37;A61K8/60;A61K8/73;A61K8/894;A61Q19/08;C08J3/07;C08J3/26;C08L5/08;A61K8/02 |
主分类号 |
C08B37/08 |
代理机构 |
Scully, Scott, Murphy & Presser, P.C. |
代理人 |
Scully, Scott, Murphy & Presser, P.C. |
主权项 |
1. A method of producing crosslinked hyaluronic acid microbeads, said method comprising:
(a) providing an aqueous alkaline solution comprising an alkali metal hydroxide and hyaluronic acid, or a salt thereof; (b) adding the aqueous solution from part (a) to an organic or oil phase to form a water in organic or water in oil (W/O) emulsion, wherein the amount of organic or oil phase used is from 20% to less than 50% by weight based on the sum of organic or oil phase and water; and (c) adding a solution comprising a crosslinking agent to the emulsion from part (b), wherein reaction of hyaluronic acid with the crosslinking agent takes place to provide crosslinked hyaluronic acid microbeads. |
地址 |
Essen DE |