Extraction method for <i>Ilex kudingcha </i>C. J. Tseng leaves, total saponins and use thereof

摘要

The present invention provides an extraction method for the leaves of Ilex kudingcha C. J. Tseng, and the total saponins from I. kudingcha leaves extracted by using this method, and use of the total saponins in preparing a drug for reducing cholesterol and blood lipids and anti-atherosclerosis. The extraction method comprises the following steps: subjecting I. kudingcha leaves to refluxing extraction with ethanol aqueous solution to obtain an extracted solution; filtering the extracted solution and removing ethanol, and then separating and purifying the solution using a macroporous resin column.

主权项

1. An extraction method for Ilex kudingcha C. J. Tseng leaves, comprising the following steps:
(1) subjecting I. kudingcha leaves to refluxing extraction with 50˜70 v/v % ethanol aqueous solution to obtain an extract solution; (2) filtering the extract solution and removing ethanol from the extract solution; (3) adsorbing the solution obtained in step (2) by a macroporous resin column, and eluting the resin column with H2O, 10˜30 v/v % alkaline ethanol aqueous solution with pH 9˜11, 10˜30 v/v % ethanol aqueous solution and 50˜70 v/v % ethanol aqueous solution, successively; and (4) collecting, concentrating and drying the eluate of 50˜70 v/v % ethanol aqueous solution to obtain an extract of I. kudingcha leaves.