Chromatographic columns with integrated electrospray emitters

摘要

A method of fabricating a chromatographic column and columns produced by such a method are disclosed. The method includes: (a) obtaining a tube comprising two open ends and a lumen with a diameter of 75 microns or less extending between the two open ends; (b) depositing a liquid silicate composition into the tube lumen at a first open end of the tube; (c) forming a porous ceramic material from the composition in the tube lumen at a location near the first open end such that a space is formed between the ceramic material and the open end that is substantially free of the ceramic material; and (d) forming a tapered emitter having an orifice diameter of less than 3 microns at the first open end.

主权项

1. A method of fabricating a chromatographic column, the method comprising:
(a) obtaining a tube comprising first and second open ends and a lumen with a diameter of 75 microns or less extending between the two open ends; (b) depositing a liquid silicate composition into the tube lumen at the first open end of the tube; (c) forming a porous ceramic material from the composition in the tube lumen at a location near the first open end and opposite to the second open end such that a space is formed that is substantially free of the ceramic material between the ceramic material and the first open end; (d) forming a sorbent bed in the tube lumen; and (e) forming a tapered emitter having an orifice with a diameter of less than 3 microns at the first open end.