Methods for producing oocysts

摘要

The present invention provides improved methods and compositions for producing oocysts. The oocysts produced according to the invention find use in the manufacture of vaccines. In preferred embodiments, the present invention provides methods and compositions for the production of Eimeria oocysts. Vaccines containing Eimeria oocysts, sporocysts and/or sporozoites produced according to the present invention may be used to immunize birds against coccidiosis either in ovo or post hatch.

主权项

1. A method of sporulating viable Eimeria oocysts: the method comprising: a. providing non-sporulated, viable Eimeria oocysts; and b. contacting the non-sporulated, viable Eimeria oocysts with a composition comprising a peroxygen compound and a combination of citric and propionic acids to effect sporulation, said composition having an acidic pH of less than pH 4 wherein said method does not include the use of potassium dichromate.