发明名称 SEPARATOR DEVICE, DEPOSITION DEVICE AND SYSTEM FOR HANDLING OF SOMATIC PLANT EMBRYOS
摘要 Methods and devices for separating fluid-suspended plant propagules and non-plant propagules based on differences in their fluid drag properties are disclosed. Deposition method and device for depositing plant propagules into a receiver comprising growth substrate by means of a fluid jet is disclosed. An automated system for processing plant propagules from a bioreactor to the growth substrate is also disclosed.
申请公布号 US2015225693(A1) 申请公布日期 2015.08.13
申请号 US201514693250 申请日期 2015.04.22
申请人 GEORGIA TECH RESEARCH CORPORATION 发明人 AIDUN Cyrus K.
分类号 C12N5/04 主分类号 C12N5/04
代理机构 代理人
主权项 1. A method of separating fluid-suspended plant propagules from non-plant propagule tissues comprising the steps of: a. providing a suitable separator container, said container containing fluid having a density lower than of the embryos to be separated, being essentially cylindrical in shape, having an essentially flat bottom wall and an essentially vertical axis, and comprising a conduit in communication with the fluid in the container at an axial region of the bottom wall during operation, and providing a rotation object positioned inside the container for inducing an axisymmetric rotating flow; b. creating a sink vortex at the axial region of the bottom wall by draining fluid from said conduit, and inducing an axisymmetric rotating flow in the fluid relative to the bottom wall by rotating the rotation object, thus: i. creating a viscous boundary layer at the bottom wall; andii. creating a radial pressure gradient in the separator container; c. introducing the fluid-suspended plant propagules and the non-plant propagule tissues to be separated into the fluid present in the separator container at a location away from the bottom wall, thus: i. sedimenting the plant propagules faster than the non-plant propagule tissues tissue;ii. allowing the plant propagules to enter the viscous boundary layer while not allowing the non-plant propagule tissues to enter the viscous boundary layer;iii. drawing the plant propagules entering the viscous boundary layer into the axial region of the bottom wall; and d. collecting plant propagules from said axial region of the bottom wall in the fluid drained from the conduit, whereby the collected are essentially separated from the non-plant propagule tissue.
地址 Atlanta GA US