发明名称 |
METHOD OF WASHING ADHERENT CELL USING TREHALOSE-CONTAINING CELL-WASHING SOLUTION |
摘要 |
Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v) %. |
申请公布号 |
US2015224145(A1) |
申请公布日期 |
2015.08.13 |
申请号 |
US201514692437 |
申请日期 |
2015.04.21 |
申请人 |
OTSUKA PHARMACEUTICAL FACTORY, INC. |
发明人 |
KOBAYASHI Eiji;Wada Tamaki;Doi Masako |
分类号 |
A61K35/28 |
主分类号 |
A61K35/28 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method of washing an adherent cell, comprising washing the adherent cell using a cell-washing solution comprising a physiological aqueous solution with trehalose or its derivative or a salt thereof added thereto, before detaching the adherent cell from a culture vessel by proteolytic enzyme treatment. |
地址 |
Naruto-shi JP |