发明名称 |
METHODS FOR PRODUCING AUTOLOGOUS T CELLS USEFUL TO TREAT B CELL MALIGNANCIES AND OTHER CANCERS AND COMPOSITIONS THEREOF |
摘要 |
<p>Provided herein are methods for manufacturing T cells. In certain embodiments, methods for manufacturing T cells which express a cell surface receptor that recognizes a specific antigenic moiety on the surface of a target cell are provided. Such methods may include, but are not limited to, steps of (1 ) enriching a population of lymphocytes obtained from a donor subject; (2) stimulating the population of lymphocytes with one or more T-cell stimulating agents to produce a population of activated T cells, wherein the stimulation is performed in a closed system using serum-free culture medium; (3) transducing the population of activated T cells with a viral vector comprising a nucleic acid molecule which encodes the cell surface receptor, using a single cycle transduction to produce a population of transduced T cells, wherein the transduction is performed in a closed system using serum-free culture medium; and (4) expanding the population of transduced T cells for a predetermined time to produce a population of engineered T cells, wherein the expansion is performed in a closed system using serum-free culture medium. Also provided herein are populations of engineered T cells produced by the methods described herein and pharmaceutical compositions thereof.</p> |
申请公布号 |
WO2015120096(A2) |
申请公布日期 |
2015.08.13 |
申请号 |
WO2015US14520 |
申请日期 |
2015.02.04 |
申请人 |
BETTER, MARC;FELDMAN, STEVEN, A.;ROSENBERG, STEVEN, A. |
发明人 |
BETTER, MARC;FELDMAN, STEVEN, A.;ROSENBERG, STEVEN, A. |
分类号 |
A61K31/549 |
主分类号 |
A61K31/549 |
代理机构 |
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地址 |
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