Micro-Liquid Phase Reaction Method Based on Substrate with Hydrophilic-Hydrophobic Patterned Surface

摘要

A micro-liquid phase reaction method based on a substrate with a hydrophilic-hydrophobic patterned surface, including the following: applying a liquid phase system containing a hydrotropic substance and/or an amphipathic substance to a hydrophobic smooth plane in a sample-spotting manner to form an array of tiny droplets, subsequently removing the solvent in each droplet to bond the hydrotropic substance and/or amphipathic substance in each droplet to the hydrophobic smooth plane so as to form an array of hydrophilic bonding points, then moving an aqueous phase system or hydrophilic liquid phase system containing more than one reactants over the hydrophobic smooth plane, thereby forming island-like tiny reaction droplets at each hydrophilic bonding point, and finally under the set reaction conditions, reacting the reactants in each tiny reaction droplet. The method allows a parallel processing system for multiple reactions to be implemented under common experiment conditions, and greatly extends the application range thereof

主权项

1. A micro-liquid phase reaction method based on a substrate with a hydrophilic-hydrophobic patterned surface, characterized by comprising the following steps:
(1) firstly providing a substrate at least a part of whose surface area is a hydrophobic smooth plane, and applying a first liquid phase system at least containing a hydrophilic substance and/or an amphipathic substance to the hydrophobic plane to form an array of tiny droplets; (2) subsequently removing the substances with larger volatility than the set reference value in each droplet to bond at least a part of the hydrophilic substance and/or the amphipathic substance or at least a part of the hydrophilic substance and/or the amphipathic substance and other substances with smaller volatility than the set reference value in each droplet to the hydrophobic smooth plane so as to form an array of hydrophilic bonding points; (3) then moving a second liquid phase system which is an aqueous phase system or a hydrophilic liquid phase system over the hydrophobic smooth plane to render the second liquid phase system automatically congregate at each hydrophilic bonding point so as to form island-like tiny reaction droplets; and (4) Finally under the set reaction conditions, reacting the reactants all of which come from the second liquid phase system or simultaneously from the first liquid phase system and the second liquid phase system in each tiny reaction droplet.