METHODS FOR THE MANUFACTURE OF PROTEOLYTICALLY PROCESSED POLYPEPTIDES

摘要

The present invention relates to a novel proteolytically active polypeptide and various uses of the polypeptide (and others) in screening and manufacturing methods.

主权项

1. A proteolytically active truncation of a precursor polypeptide, wherein said precursor polypeptide consists of a polypeptide having at least 90% sequence identity with SEQ ID NO: 2, wherein said proteolytically active truncation lacks one or more amino acid residues N-terminal to amino acid position 249 of SEQ ID NO: 2, and wherein said proteolytically active truncation is capable of hydrolysing botulinum or tetanus neurotoxin to produce di-chain botulinum or di-chain tetanus neurotoxin.