发明名称 |
LOW OXYGEN TENSION ENHANCES ENDOTHELIAL FATE OF HUMAN PLURIPOTENT STEM CELLS |
摘要 |
Low oxygen tension is a critical regulator of the developing or regenerating vasculature. The present invention is based on the determination that low oxygen tension during early stages of early vascular cell (EVC) derivation induces endothelial commitment and maturation of pluripotent stem cells. Inhibition of reactive oxygen species generation during the early stages of differentiation abrogates the endothelial inductive effects of the low oxygen environments. Methods of generating various types of cells from pluripotent stem cells (PSCs) are described, as well as compositions and methods of use thereof. In particular, generation of EVCs, bicellular vascular populations, early endothelial cells (ECs) and pericytes via culture in a low oxygen environment is described. |
申请公布号 |
WO2015119642(A1) |
申请公布日期 |
2015.08.13 |
申请号 |
WO2014US30616 |
申请日期 |
2014.03.17 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
GERECHT, SHARON;KUSUMA, SRAVANTI |
分类号 |
C12N5/071;A61K35/12;C12N5/0789 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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地址 |
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