METHOD FOR DETECTING HIV

摘要

The present invention provides a semi-qualitative method for testing detectable and undetectable HIV-1 viral load of HIV-1 positive patients. The method makes use of the RT- LAMP assay (Reverse Transcriptase Loop Mediated Isothermal Amplification) and hydroxy- napthol blue (HNB), a colour indicator, to visually indicate whether the HIV viral load of the patient being tested is above or below a certain threshold. Primer sets for use in the method have been designed which amplify a target in the integrase region of the pol gene of HIV-1, the gag gene of HIV-1, or the Long Terminal Repeat (LTR) region of HIV-1.