发明名称 |
Probe for detecting polymorphism in CYP3A gene, method of detecting polymorphism, method of evaluating drug efficacy, and reagent kit for detecting polymorphism |
摘要 |
<p>Provided is a probe for detecting polymorphism that enables a simple detection of polymorphism in the CYP3A gene with high sensitivity. A probe which detects polymorphism in the CYP3A gene, the probe including:
(P1) a fluorescently labeled oligonucleotide having a sequence including the 401st to the 411th bases of the sequence indicated in SEQ ID NO: 1 and having a length of 11 bases to 50 bases, the sequence having at least 80% identity with respect to a base sequence having the same bases as SEQ ID NO: 1 with the exception that the base corresponding to the 411th base in SEQ ID NO: 1 is cytosine, and the base corresponding to the 411th base of the sequence indicated in SEQ ID NO: 1 is labeled with a fluorescent dye; or
(P1') a fluorescently labeled oligonucleotide having a sequence including the 401st to the 411th bases of the sequence indicated in SEQ ID NO: 1 and having a length of 11 bases to 50 bases, the sequence being capable of hybridization under stringent conditions to the complementary strand of a base sequence having the same bases as SEQ ID NO: 1 with the exception that the base corresponding to the 411th base in SEQ ID NO: 1 is cytosine, and the base corresponding to the 411th base of the sequence indicated in SEQ ID NO: 1 is labeled with a fluorescent dye.</p> |
申请公布号 |
EP2497835(B1) |
申请公布日期 |
2015.08.12 |
申请号 |
EP20120158915 |
申请日期 |
2012.03.09 |
申请人 |
ARKRAY, INC. |
发明人 |
IGUCHI, AKI;HIRAI, MITSUHARU |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|