Techniques are described for quantification of molecules in a sample. Mass spectrometry is performed to obtain ionization intensities for precursor and product ions originating from a particular molecule. A first stet of precursor ions having the highest ionization intensities and originating from the particular molecule is determined. For each of the one or precursors in the first set, determined is a second set of one or more product ions that are fragments associated with said each precursor and have the highest ionization in intensities of product ions associated with said each precursor. An intensity sum is calculated for the particular molecule by adding ionization intensities of product ions included in the second sets for the one or more precursors in the first set. The intensity sum is compared to information included in a calibration standard. A quantity of the particular molecule in the sample is determined based on said comparing.
申请公布号
EP2786130(A4)
申请公布日期
2015.08.12
申请号
EP20120853989
申请日期
2012.11.16
申请人
WATERS TECHNOLOGIES CORPORATION
发明人
GEROMANOS, SCOTT, J.;VISSERS, JOHANNES PC;LANGRIDGE, JAMES, I.