| 发明名称 | 产前染色体数目快速检测试剂盒 | ||
| 摘要 | 本发明公开了产前染色体数目快速检测试剂盒中的13号，21号，18号，X和Y染色体特异性探针序列，以及对快速检测的实验步骤进行细致的描述。这种快速检测的方法大大提高了荧光原位杂交检测效率，有利于临床的诊断及治疗。 | ||
| 申请公布号 | CN104830980A | 申请公布日期 | 2015.08.12 |
| 申请号 | CN201510211520.1 | 申请日期 | 2015.04.29 |
| 申请人 | 广州和实生物技术有限公司 | 发明人 | 陈华云;刘淑园;陈嘉昌;丁渭;肖湘文;黄爽;曾烨;赵丽;方凤银 |
| 分类号 | C12Q1/68(2006.01)I | 主分类号 | C12Q1/68(2006.01)I |
| 代理机构 | 代理人 | ||
| 主权项 | 产前染色体数目快速检测试剂盒，包括FISH荧光探针，缓冲液和染色液，其中，探针的序列分别为：18号染色体特异性探针序列：<tables num="0001" id="ctbl0001"><table><tgroup cols="2"><colspec colname="c001" colwidth="21%" /><colspec colname="c002" colwidth="78%" /><tbody><row><entry morerows="1">探针1</entry><entry morerows="1">TCTTCAGATAAAATCTAGACAGA</entry></row><row><entry morerows="1">探针2</entry><entry morerows="1">CCTACGTTGGAAAAGGAAATATCT</entry></row><row><entry morerows="1">探针3</entry><entry morerows="1">TTGAAACACTCTTTTTGTAGAATC</entry></row><row><entry morerows="1">探针4</entry><entry morerows="1">CATTCTCAGAAACTTGTTTATGCTG</entry></row><row><entry morerows="1">探针5</entry><entry morerows="1">CTATCATAGAGCAGGTTGGAATCAC</entry></row><row><entry morerows="1">探针6</entry><entry morerows="1">CAAGAGGATATTTGCATAGCTTTG</entry></row><row><entry morerows="1">探针7</entry><entry morerows="1">CATTCCCTTTCACAGAGCAGGTTT</entry></row><row><entry morerows="1">探针8</entry><entry morerows="1">CATAACAACTAGACACAAGCATTC</entry></row><row><entry morerows="1">探针9</entry><entry morerows="1">CTGGACTTTTGGAGCGATTTCAGG</entry></row><row><entry morerows="1">探针10</entry><entry morerows="1">CTTACTCGTGATGTGTGTCCTCAAC</entry></row><row><entry morerows="1">探针11</entry><entry morerows="1">TAGAGCAGGTTTGAAACACTCTT</entry></row><row><entry morerows="1">探针12</entry><entry morerows="1">CAACTAACAAAGTTGAACCTTTCT</entry></row><row><entry morerows="1">探针13</entry><entry morerows="1">CTGGACAGAAGCATTCTCAGAAA</entry></row><row><entry morerows="1">探针14</entry><entry morerows="1">AGTATCTGGAACTGGACTTTTGG</entry></row><row><entry morerows="1">探针15</entry><entry morerows="1">TAAGAGAATTGAACCACCGTTT</entry></row><row><entry morerows="1">探针16</entry><entry morerows="1">TGAGAAACTGCTTTCTGATGTTTG</entry></row></tbody></tgroup></table></tables>X染色体特异性探针序列：<tables num="0002" id="ctbl0002"><table><tgroup cols="2"><colspec colname="c001" colwidth="21%" /><colspec colname="c002" colwidth="78%" /><tbody><row><entry morerows="1">探针1</entry><entry morerows="1">CAAACGGAAGCATTCTCAGAATAT</entry></row><row><entry morerows="1">探针2</entry><entry morerows="1">CAGTTTCCAAATACACTTTTGGTAG</entry></row><row><entry morerows="1">探针3</entry><entry morerows="1">CTAAACTGAAGCATTCTCAGAAAC</entry></row><row><entry morerows="1">探针4</entry><entry morerows="1">CTCTTTGAAGATTTCTTTGGAAACG</entry></row><row><entry morerows="1">探针5</entry><entry morerows="1">TGGACCTCTTTGAAGGTTTCG</entry></row><row><entry morerows="1">探针6</entry><entry morerows="1">CAGCAGTTTCGAAACACTCTTTCT</entry></row><row><entry morerows="1">探针7</entry><entry morerows="1">CTTCACCTAAAAGCTAAACGG</entry></row><row><entry morerows="1">探针8</entry><entry morerows="1">CCTTTGATAGCGCAGCTTTGACAC</entry></row><row><entry morerows="1">探针9</entry><entry morerows="1">CAGAAACTTCTTTGGGATGTTTG</entry></row><row><entry morerows="1">探针10</entry><entry morerows="1">CGACATAGAAGCATTCTCAGAA</entry></row><row><entry morerows="1">探针11</entry><entry morerows="1">CTGCTCTGTGATGATTGCATTCAA</entry></row></tbody></tgroup></table></tables>Y染色体特异性探针序列：<tables num="0003" id="ctbl0003"><table><tgroup cols="2"><colspec colname="c001" colwidth="21%" /><colspec colname="c002" colwidth="78%" /><tbody><row><entry morerows="1">探针1</entry><entry morerows="1">CATCTCACGGAGTTGAACCTT</entry></row><row><entry morerows="1">探针2</entry><entry morerows="1">CACCTCACAGACTTGAAACTT</entry></row><row><entry morerows="1">探针3</entry><entry morerows="1">CTTTCAGGCCTATGGTGGAAAAGG</entry></row><row><entry morerows="1">探针4</entry><entry morerows="1">CTGCAAGTGGATATGGAGAGAGCT</entry></row><row><entry morerows="1">探针5</entry><entry morerows="1">TGGAGCCTTTTGTGGCCTACG</entry></row><row><entry morerows="1">探针6</entry><entry morerows="1">TACTACACAGAAGCATTCTGAG</entry></row><row><entry morerows="1">探针7</entry><entry morerows="1">CACAGACGCATTCTGAGAAACTTC</entry></row><row><entry morerows="1">探针8</entry><entry morerows="1">CTCTTGATCGAGTAGTTTTGAACCT</entry></row><row><entry morerows="1">探针9</entry><entry morerows="1">CATCTCACAGAGTTGAACCTTTCT</entry></row><row><entry morerows="1">探针10</entry><entry morerows="1">CATTTGACAGAGCGGTTTTG</entry></row><row><entry morerows="1">探针11</entry><entry morerows="1">TAAGGTGGGAAAGGAAATATCTTC</entry></row></tbody></tgroup></table></tables>21号染色体特异性探针序列：<tables num="0004" id="ctbl0004"><table><tgroup cols="2"><colspec colname="c001" colwidth="21%" /><colspec colname="c002" colwidth="78%" /><tbody><row><entry morerows="1">探针1</entry><entry morerows="1">TAGAATCTGCGAGGGGATATTTG</entry></row><row><entry morerows="1">探针2</entry><entry morerows="1">CGACAGAAGCATTCTCAGAAACT</entry></row><row><entry morerows="1">探针3</entry><entry morerows="1">TGAAACACTCTTTTTGTAGTATC</entry></row><row><entry morerows="1">探针4</entry><entry morerows="1">CTACGGTGAAAAGGGAAATAT</entry></row><row><entry morerows="1">探针5</entry><entry morerows="1">TGGATATTTGGATAGCTTGGAGG</entry></row><row><entry morerows="1">探针6</entry><entry morerows="1">CAGAGTTGAACATTCCCTTTC</entry></row><row><entry morerows="1">探针7</entry><entry morerows="1">CTAACAGAGTTGAACCTTTCT</entry></row><row><entry morerows="1">探针8</entry><entry morerows="1">TGACAGAGCAGTTTTGAAACA</entry></row><row><entry morerows="1">探针9</entry><entry morerows="1">CGGGAATATCTTCATATCAAATCT</entry></row><row><entry morerows="1">探针10</entry><entry morerows="1">CATTCAAGTCACAGAGTTGAATAT</entry></row><row><entry morerows="1">探针11</entry><entry morerows="1">CGTTGGAAACGGGAATATCTTC</entry></row><row><entry morerows="1">探针12</entry><entry morerows="1">CATTCCCTTTCGTACAGCAGTTT</entry></row><row><entry morerows="1">探针13</entry><entry morerows="1">TGCATTCAACTCATAGAGTTGA</entry></row></tbody></tgroup></table></tables>13号染色体探针由BAC载体传统方法合成。 | ||
| 地址 | 510665 广东省广州市经济技术开发区科学城荔枝山路6号1号楼510室 | ||