发明名称 |
IMPROVED PRODUCTION HOST CELL LINES |
摘要 |
<p>The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a DHFR expression cassette. Those cell lines have improved growth characteristics in comparison to DHFR-deficient or DHFR-reduced cell lines such as CHO DG44 and CHO DUKX-B11. The invention especially concerns two cell lines, a representative of each cell line is deposited with the DSMZ under the number DSM ACC2909 (CHOpper® Discovery) and DSM ACC2910 (CHOpper® Standard). The invention further concerns a method of producing proteins using the cells generated by the described method.</p> |
申请公布号 |
EP2313497(B1) |
申请公布日期 |
2015.08.12 |
申请号 |
EP20090800056 |
申请日期 |
2009.07.22 |
申请人 |
BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG |
发明人 |
KAUFMANN, HITTO;FLORIN, LORE;BECKER, ERIC;STUDTS, JOEY M. |
分类号 |
C12N9/06;C12N15/63;C12N15/85 |
主分类号 |
C12N9/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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