| 摘要 |
The present invention relates to a method for purifying nucleic acids using a nucleic acid-binding phase which is furnished with a deficit of nucleic acid-binding groups A having a pK of 8 to 13, or which has groups A and binding-inhibiting groups N which are neutrally charged during the binding, and preferably during the elution, and the method comprises the following steps: (a) binding the nucleic acids to the nucleic acid-binding phase at a pH which is below the pH of the pK of the nucleic acid-binding groups A (binding pH); (b) eluting the nucleic acids at a pH which is above the binding pH (elution pH). In addition, corresponding kits and also nucleic acid-binding phases which can be used for purifying nucleic acids are disclosed. The technology according to the invention permits the purification of nucleic acids and, in particular, elution, with use of low salt concentrations, and so the purified nucleic acids can be directly processed, for example used in a PCR. |
| 主权项 |
1. A method of purifying nucleic acids comprising:
(a) binding the nucleic acids to a nucleic acid-binding phase at a binding pH, the nucleic acid binding phase comprising nucleic acid binding groups having a pKa of from 8 to 13 and charge neutral groups having a neutral charge at the binding pH, wherein the binding pH is below the pKa of the nucleic acid-binding groups; and (b) eluting the nucleic acids at an elution pH which is above the binding pH,
(i) wherein the nucleic acid biding groups are compounds selected from the group consisting of: whereinn is 1 to 5R is a C1 to C6 alkyl group; and* is amino, aminomethyl, aminoethyl, aminopropyl, dimethylamino, diethylamino, diisopropylamino, dipropylamino, diethanolamino, dipropanolamino, diethylenetriamine, triethylenetetramine, tetraethylenepentamine, etheramine, polyetheramine, 4-diisobutylamino-1-butane, or 6-dipropylamino-1-hexane, and(ii) wherein the charge neutral groups are compounds selected from the group consisting of whereinn is 1-5R is a C1 to C6 alkyl group;* is hydroxymethyl, hydroxyethyl, hydroxypropyl, ethanediol, propanediol, propanetriol, butanetriol, 3-glycidoxypropyl, ethyl glycidyl ether, or a C1 to C4 alkyl radical, or halogen. |
