| 发明名称 | Orthogonal translation components for the in vivo incorporation of unnatural amino acids | ||
| 摘要 | The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems. | ||
| 申请公布号 | US9102932(B2) | 申请公布日期 | 2015.08.11 |
| 申请号 | US201213492296 | 申请日期 | 2012.06.08 |
| 申请人 | The Scripps Research Institute | 发明人 | Schultz Peter G.;Alfonta Lital;Chittuluru Johnathan R.;Deiters Alexander;Groff Dan;Summerer Daniel;Tsao Meng-Lin;Wang Jiangyun;Wu Ning;Xie Jianming;Zeng Huaqiang;Seyedsayamdost Mohammad;Turner James |
| 分类号 | C12P21/06;C12N9/00;C12P21/02;C12N15/10;C12P21/00 | 主分类号 | C12P21/06 |
| 代理机构 | Quine Intellectual Property Law Group, P.C. | 代理人 | Quine Intellectual Property Law Group, P.C. |
| 主权项 | 1. A translation system comprising: (a) a first unnatural amino acid which is p-(2 amino-1-hydroxyethyl)-L-phenylalanine; (b) a first orthogonal aminoacyl-tRNA synthetase (O-RS); and (c) a first orthogonal tRNA (O-tRNA); wherein said first O-RS preferentially aminoacylates said first O-tRNA with said first unnatural amino acid; and wherein the first O-RS comprises at least 90% identity to the amino acid sequence of SEQ ID NO 59. | ||
| 地址 | La Jolla CA US | ||