| 主权项 |
1. A process for preparing a compound of structural formula (1) in enantiomeric excess,wherein
each ring A and B is independently an optionally substituted monocyclic aryl or heteroaryl, R1 is selected from hydrogen, hydroxy, halo, cyano, carboxy, and an optionally substituted alkyl, aminoalkyl, cycloalkyl, heterocycloalkyl, alkyloxycarbonyl, aminocarbonyl, aminothiocarbonyl, aminosulfonyl, and sulfonyl; the process comprising contacting the compound of structural formula (2), wherein A and B, and R1 are defined above, with a ketoreductase polypeptide comprising an amino acid sequence that has at least 80% sequence identity to the reference polypeptide of SEQ ID NO: 10, wherein the amino acid sequence comprises X80 is T; X96 is V or R; X145 is L; X153 is T; X190 is P; X196 is L or M; and X226 is V, wherein the ketoreductase is capable of converting compound (2) to compound (1) in enantiomeric excess, in presence of NADH or NADPH under suitable reaction conditions. |
