Image processing device, imaging device, computer-readable storage medium, and image processing method

摘要

A reflected light image and a fluorescent light image of an observed region are acquired with an imaging system. A gradation level of the reflected light image is set on the basis of a feature value of the acquired reflected light image. The focus of the imaging system is controlled in accordance with the set gradation level. The fluorescent light image is corrected using a focus-controlled reflected light image of the observed region. Accordingly, the image of an object in the observed region from which the fluorescent light is generated is sharpened.

主权项

1. An image processing device, comprising:
an image acquiring unit that acquires a reflected light image and a fluorescent light image of an observed region, the reflected and the fluorescent light images being captured by an imaging system; a feature value calculating unit that calculates a feature value of the reflected light image acquired by the image acquiring unit; an amount-of-shift-of-focus setting unit that sets an amount of shift of focus of the reflected light image on the basis of the feature value; a control unit that controls a focus of the imaging system in accordance with the amount of shift of focus; and a corrected fluorescent light image generating unit that generates a corrected fluorescent light image that is obtained by correcting the fluorescent light image using a focus-controlled reflected light image of the observed region that is acquired by the image acquiring unit when the focus is controlled to be in an unfocused state; wherein the control unit controls the focus so that the focus-controlled reflected light image arrives at the unfocused state in the observed region in accordance with the amount of shift of focus; wherein the imaging system includes: a reflected light imaging unit that captures the reflected light image with the focus being on the observed region; and a fluorescent light imaging unit that captures the fluorescent light image with the focus being on the observed region; wherein the reflected light imaging unit captures the focus-controlled reflected light image after the focus is controlled in accordance with the amount of shift of focus.