| 发明名称 | Trehalose phosphorylases and their use in the biocatalytic production of trehalose-analogues and glycosyl phosphates | ||
| 摘要 | The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogs and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity. | ||
| 申请公布号 | US9102922(B2) | 申请公布日期 | 2015.08.11 |
| 申请号 | US201113885789 | 申请日期 | 2011.12.09 |
| 申请人 | Universiteit Gent | 发明人 | Van Der Borght Jef;Desmet Tom;Soetaert Wim;Chen Chao |
| 分类号 | C12N9/10;C12P19/12;C12P19/18;C12P19/02 | 主分类号 | C12N9/10 |
| 代理机构 | Clark & Elbing LLP | 代理人 | Clark & Elbing LLP |
| 主权项 | 1. A mutated trehalose phosphorylase comprising the amino acid sequence of SEQ ID NO: 1, except for a mutation in one or more of amino acid positions 371, 442, 450, 649, 659, or 693, and wherein said mutated trehalose phosphorylase has an increased catalytic efficiency towards its acceptor or donor substrate compared to the corresponding wild-type trehalose phosphorylase. | ||
| 地址 | Ghent BE | ||