Device and methods for detecting analytes in saliva

摘要

The invention provides a device for detecting drugs of abuse or other compounds in saliva. The invention thus provides a device for detecting the presence of one or more analytes in a saliva sample, comprising: (a) One or more pre-treatment regions for specifically or non-specifically removing at least a part of the fraction of the saliva sample interfering with detection of the one or more analytes; and (b) A detection region comprising a biosensor surface, the surface comprising: molecules capable of specifically binding the one or more analytes; or the one or more analytes and/or analyte analogs.

主权项

1. A microfluidic device for detecting the presence of one or more analytes in a saliva sample, comprising:
one or more pre-treatment chambers comprising a surface which comprises a first molecule capable of specifically or non-specifically binding to one or more saliva components in the saliva sample interfering with the detection of the one or more analytes for specifically or non-specifically removing at least a part of the fraction of components in the saliva sample interfering with detection of the one or more analytes, wherein the first molecule capable of specifically or non-specifically binding to one or more saliva components in the saliva sample interfering with detection of the one or more analytes is hydroxyapatite or an anti-mucin antibody; and a detection chamber separate from and fluidically coupled to the one or more pre-treatment chambers and comprising a biosensor surface, the biosensor surface comprising:
second molecules capable of specifically binding the one or more analytes; orthe one or more analytes and/or second molecules which are structurally related to the analyte and bind analyte-specific probes.