| 发明名称 |
Cellular permissivity factor for viruses and uses thereof |
| 摘要 |
The present invention provides methods and compositions related to the generation of host cells permissive for virus growth, particularly Porcine Reproductive and Respiratory Syndrome (PRRS) virus. |
| 申请公布号 |
US9102912(B2) |
申请公布日期 |
2015.08.11 |
| 申请号 |
US201313940317 |
申请日期 |
2013.07.12 |
| 申请人 |
Zoetis Services LLC |
发明人 |
Calvert Jay Gregory;Shields Shelly;Slade David E.;Welch Siao-Kun |
| 分类号 |
C12N7/02;A61K39/12;C12N7/00;C07K14/705;C12N5/071;A61K39/00 |
主分类号 |
C12N7/02 |
| 代理机构 |
|
代理人 |
Renda Barbara L.;Donahue E. Victor |
| 主权项 |
1. A method of facilitating production of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) from a culture of vertebrate cells, comprising the steps of:
(a) providing a recombinant vertebrate cell transfected with an exogenous polynucleotide that encodes a polypeptide having a transmembrane domain and at least 85% sequence identity to SEQ ID NO:46, so that expression of CD163 polypeptide in said cell is increased; (b) contacting a culture of said cell with PRRSV virus under conditions which permit infection of the cells and growth of the virus; and (c) recovering virus from said culture. |
| 地址 |
Florham Park NJ US |
