Optically accessible microfluidic diagnostic device

摘要

A microfluidic diagnostic device (1, 50) comprising a substrate (4); a compatible layer (6) formed on a first face (4a) of the substrate (4); a structural layer (8), formed on top of the compatible layer (6); a channel (10), formed in the structural layer (8) and limited underneath by the compatible layer (6), optically accessible by a first luminous radiation having a first wavelength (λe); and a cover layer (18) made of a material transparent to the first wavelength (λe), arranged on top of the structural layer (8) and sealing the channel (10) at the top, wherein the compatible layer (6) has a thickness equal to approximately a quarter of the first wavelength (λe) divided by the refraction index of the compatible layer (6), or equal to an odd multiple of a quarter of the first wavelength (λe) divided by the refraction index of the compatible layer (6).

主权项

1. A microfluidic diagnostic device comprising:
a substrate; a compatible layer formed on a upper face of the substrate and having a refraction index; a structural layer, formed on top of the compatible layer; a channel, formed in the structural layer and limited underneath by the compatible layer, said channel being optically accessible from above by a first luminous radiation having a first wavelength (λe); and an inlet hole and an outlet hole each extending through the substrate and the compatible layer and in microfluidic connection with said channel, said inlet hole and said outlet hole formed starting from a bottom, external face of said microfluidic diagnostic device, opposite to an optically accessible top face,
said compatible layer being substantially transparent to the first wavelength (λe) and having a thickness equal to approximately a quarter of the first wavelength (λe) divided by the refraction index of said compatible layer, or equal to an odd multiple of a quarter of the first wavelength (λe) divided by the refraction index of said compatible layer; andthe compatible layer being arranged in such a way that the incidence angle of the incident light radiation is approximately orthogonal to the surface of the compatible layer.